Coding

Part:BBa_K2074023:Design

Designed by: Yi Cai   Group: iGEM16_FAFU-CHINA   (2016-10-09)


Cry11Aa(Codon optimization)-Extended FMDV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal SapI.rc site found at 1440


Design Notes

To identify there is no restriction enzyme cutting site which we choosed.


Source

DNA from a synthesis company.

References